"The schools have a serious lack of resources, and the counselors can be swamped with disciplinary problems and just getting kids to graduation," said Reginald Hillmon, who manages the transcript evaluation system.
The Enzo BioArray High Yield RNA Transcript Labeling System (Enzo) was used for labeling the sample prior to hybridization.
The group of Breyne et al. [ 7] recently published an attempt to further develop cDNA-AFLP technique into a quantitative genome-wide transcript profiling system.
Samples were labeled with the Affymetrix Whole Transcript Labeling system and then hybridized to the Affymetrix GeneChip Rat Gene 1.0 ST Array (Santa Clara, CA).
mRNA differential display (DDRT-PCR) [ 4], cDNA-AFLP [ 5] and different variants of these two methods [ 6] are gel-based transcript profiling systems based on electrophoretic fingerprinting of amplified cDNA fragments.
Proprietary high-end transcript profiling systems such as Massive Parallel Signature Sequencing [ 1] and GeneCalling [ 2] are excellent tools for genome-wide quantitative transcription analysis on any organism, but they are available to only a small fraction of researchers.
For each of these transcript definition systems, the probes that could be uniquely assigned to their transcript targets were assembled in custom Channel Definition Files (CDF files) as individual probe sets, which assumed the names of the matched transcripts, thus replacing the Affymetrix probe set ID's.
For synthesis of uncapped T7 transcripts, RiboMAX Large Scale RNA Production System-T7 (Promega) was used according to the manufacturer's protocols.
Figure 1 shows a screenshot of MAXqda with a list of imported transcripts, the code system in English, and a transcript with coded segments.
In doing so, we can model the concentration of the morphogen's mRNA transcripts in the system and equate the corresponding mRNA transcript concentration profile to that of the morphogen protein concentration profile.
