Despite of a slight bias generated during NSR hexamer priming, the NSR-seq method still robustly reproduces the same transcript sites in different samples.
Except for the 96 miRNAs with unknown transcript sites, miR4250 had the most transcript sites with 2,352.
Through comparison with the pear genome, the transcript sites for these miRNAs were located.
From these, 266 miRNAs were located on chromosomes or scaffolds, and multiple transcript sites for all 266 miRNAs ensured the miRNA gene expression and important regulation function.
Although the present study does not address whether or not the response of editing to temperature is adaptive in Drosophila, editing in a number of the potassium channel transcript sites in this study is known to affect channel function.
Nuclear RNA samples derived from WT cells showed some evidence of transcripts close to the TSS (site a) and transcripts of pre-mRNA (site b) but barely detectable spliced transcripts (site c) in contrast to Lsh−/− samples (Fig. 5C).
This difference suggests that the release of mut transcripts from the transcription site is not as efficient as the release of wt transcripts.
Knocking-down the exosome subunit Rrp6 improved the release of the mut transcripts from the transcription site.
Counting transcripts at the transcription site.
