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This situation exists for the following reasons: i) a transcript is incomplete; ii) a transcript represents a pseudogene; iii) a transcript represents a novel gene product, but contains a domain common to an existing non-homologous protein.
To fully pursue individual splice variants requires more validation experiments, but having a profile across the transcript represents a first approximation.
The locus XLOC_014395 (with TCONS_00029058 as the longest transcript) represents a novel protein coding gene on BTA19 not previously annotated in the bovine genome assembly.
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Sequence analysis evidenced that the isolated transcript represented a typical fatty-acyl desaturase gene.
Okoniewski and Miller [ 1] reported evidence they believe to be in favor of the idea that spurious positive correlations induced by the process of multiple targeting, i.e. the competition of multiple probe sets for a common transcript, represent a mass phenomenon in high-density oligonucleotide microarrays.
Even so, our high confidence contig set of 15,527 transcripts represents a marked expansion of the An.
The RNA-seq data we used to identify novel transcripts represents a human PFC developmental time-series.
Assuming R. microplus has a similar number of transcripts as these two arthropods, the BmiGI set of 13,643 unique transcripts represents a significant fraction of the likely set of protein-coding transcripts in R. microplus.
Although a precise estimation of transcriptome coverage is unachievable without the full genome information, our collection of unique transcripts represents a substantial percentage of the genes from the genome of B. tabaci.
Four of the five predicted FLAG MKRN1-associated mRNAs exhibited mean FLAG MKRN1-associateds above threshold (Fig 4E), collectively suggesting that this cluster of transcripts represents a mRNAs-mexhibitedosttranscriptional gene regulatory meanle in ESCs.
Another question that can be addressed through comparative genomics is the extent to which a given collection of transcripts represents an exhaustive transcriptome.
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