Since most of the TILs show transcript patterns associated with activation37, we determined whether Chop is induced after T cell stimulation.
Typically, transcript patterns of these redundant genes are highly correlated.
Our results indicate that transcript patterns in response to insect herbivory and wounding are distinct though partially overlapping.
To compare transcript patterns with protein profiles (Fig. 1), RT-PCR experiments were performed using gene-specific primers.
Since we found very similar transcript patterns between A. thaliana and B. divaricarpa across a variety of different experimental treatments, we focused this analysis on B. divaricarpa.
These include psbD, psbD2, psbQ, psbW, psb27 and psb29, which displayed significant changes in their transcript patterns with maximum values at 06 30 or 07 30 (Fig. 5).
By contrast, De Vos et al. found that there was, in general, fairly little overlap in Arabidopsis transcript patterns in response to insects with different feeding modes [35].
In our study, we investigated plant transcript patterns in response to two further lepidopteran herbivores, the specialist P. xylostella, and the generalist T. ni.
We investigated Boechera divaricarpa transcript patterns in response to two lepidopteran herbivores, the specialist diamondback moth (Plutella xylostella), and the generalist cabbage semilooper (Trichoplusia ni).
We used these data to estimate the number of genes that showed a) differences in mean transcript level between species, and b) differences in transcript patterns between species.
Reymond et al. [22] investigated A. thaliana transcript patterns in response to two different herbivorous lepidopterans, the specialist P. rapae and the generalist S. littoralis, and they identified more than 100 insect-responsive genes.
