The activation of a paternally inherited TP therefore likely involves a posttranscriptional event that allows its transcripts—or transcripts that contain its sequence to be processed into P-element piRNAs.
When N transcripts (or transcript tags) are sequenced from a transcriptome of a given cell type, let fx be the number of unique transcripts that are detected x times.
Each column lists the relative number of transcripts or "transcript fingerprint" more than two-fold over- or under-represented within each functional category by each specific tissue type.
More than half of the sequences (11,134) had no BLAST result, indicating a high number of lepidopteran or species-specific transcripts or transcript parts (orphan UTRs) [ 23].
Contig branch structures within each isogroup were then traversed to create 1,658 isotigs (1.4 Mb), which represent unique assembled transcripts or transcript fragments.
Of the remaining 5,701 contigs, 762 (13.4%) lacked an open reading frame and were therefore possibly non-coding transcripts or transcript fragments.
To determine splicing variations in each tissue, we first mapped reads to the reference genome and assembled them into transcripts or transcript fragments.
A large proportion has no significant sequence alignment or hits in any of the databases, comprising 13,349 sequences or 24.5% of total transcripts which suggests that they may contain novel sequences or a high number of Suaeda fruticosa specific transcripts or transcript portions such as orphan untranslated regions.
These multiple transcript unigene clusters can represent transcription variants, allelic variants, closely related paralogues, misassembled transcripts, or transcripts that were fragmented due to low coverage.
The multiple contigs that were assembled into unigene clusters may represent transcription variants, allelic variants, closely related paralogous sequences, misassembled transcripts, or transcripts that were fragmented due to low coverage [ 46].
