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If the "transcript" option is selected, the table also contains other transcripts associated with a specific gene according to the described multi-transcript protocol.
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The de novo assembly was performed by the CLC Genomics Server using the scaffolding option and the mapping reads back to transcripts option.
In the Transcript assembly and abundance estimation section the user can allow the reconstruction of novel-transcripts (e.g. enable Novel-Transcripts option to adopt the RABT assembler to try to reconstruct novel transcripts supported by alignment data).
For genes where available pearl millet EST sequence was not helpful for primer design, sorghum and maize ESTs (identified using all transcripts on option in the TIGR Rice Genome Annotation Project portal) were used to design appropriate primers.
Reads from each tissue were mapped and quantified separately, using a gff3 file of peach MADS-box gene models as a reference and without assembly of additional transcripts (−G option in Cufflinks).
Click the option "Transcript file" and then name it.
Since the objective of this study was only to assemble the transcripts, the genomic option was used.
Running the pipeline with default options gives us expression estimates for known transcripts, but other option settings make different types of analysis possible.
A second internal ppm1 TSS is present in all strains at position 2309159; the resulting transcript again provides the option of dissociating the two enzymatic activities.
Both options estimate transcript abundance at close and more distant positions towards the 3′-end based on the hybridization signal [ 15, 16].
One such option is transcripts per million (TPM), implemented in the RSEM software package [ 18] and used to compute gene expression values in, e.g., The Cancer Genome Atlas [ 19].
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