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The control plasmid for monitoring transfection efficiency is MT-RLuc [ 40]. miRNA plasmids were constructed by cloning a DNA segment containing the predicted primary transcript of each miRNA into the BamHI/EcoRI site of ActMSI [ 40].
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To study the expression dynamics of miRNAs and their potential roles in gene expression regulation in S. lycopersicum and S. habrochaites, the transcript abundance of each miRNA was evaluated by transcripts per million (TPM).
1, 2 Nuclear RNase III Drosha cleaves primary transcripts of miRNA (pri-miRNAs) to yield the hairpin-structure precursor miRNAs (pre-miRNAs).
We successfully recover the majority of annotated miRNAs and transfer RNAs (tRNAs); the long hairpins of processed primary transcripts of miRNA (pre-miRNA) and four stems of tRNAs make both relatively easy for our method to detect.
Similar to protein-coding genes and some lncRNAs, primary transcripts of miRNA (pri-miRNA) genes are transcribed by RNA polymerase II (Pol II) [55].
LAGI02 contains sequences with homology to known primary transcripts of miRNAs (pri-miRNAs) and allowed us the identification of corresponding pre-miRNA hairpin structures and their mature miRNA sequence.
The primary transcripts of miRNAs (pri-miRNAs) contain 5'-m7G cap structures as well as 3'-end poly (A) tails, which are unique properties of class II gene transcripts.
We have been able to identify several primary transcripts of miRNAs (pri-miRNAs) expressed in the white lupin cluster root.
Owing to the double-stranded structure, the primary transcripts of miRNAs (pri-miRNAs) are therefore potential substrates for A-to-I RNA editing [ 44].
The primary transcripts of miRNAs (pri-miRNAs) are transcribed by RNA polymerase II, and processed into hairpins by Drosha-DGCR8 the microprocessor, then exported to the cytoplasm by Exportin5.
The relative abundance of each miRNA transcript was then determined using the comparative Ct method [28].
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