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Only the first listed alternative transcript of each gene was considered.
We annotated the assembled genes by selecting the longest transcript of each gene.
The longest transcript of each gene was used for the analyses.
For calculations of RPKM, the length of the longest transcript of each gene was used.
This annotation is based on phylogenetic analyses of clusters of homologous sequences corresponding to the longest transcript of each gene.
Primers were designed to amplify regions of exons present in the longest annotated transcript of each gene.
Similar(41)
The molar amounts of transcripts of each gene were calculated based on crossing point analysis, with standard curves generated from standard cDNA.
In vitro transcripts of each gene were generated as standards.
The numbers of associated transcripts of each gene were normalized and logarithmically transformed for comparisons purposes.
Relative transcripts of each gene were expressed as mean ± standard error.
All subsequent analysis was done only for main transcripts of each gene.
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