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RNA, a more ancient chemical version of DNA, performs many basic tasks in a cell, one of which is to form a copy or transcript of a gene and direct the synthesis of the gene's protein.
The initial transcript of a gene is processed by a delicate piece of cellular machinery known as a spliceosome, which strips out all the introns and joins the exons together.
Every known or predicted form of transcript of a gene will be assigned a systematic identifier that will be the same as the locus identifier except that the letter 'g' for gene will be replaced by letter 't' for transcript, to be added as a suffix following the two-digit chromosome identifier.
The specificity of all qPCR assays was verified by testing for detection of a standard known to contain only the full-length transcript of a gene with the qPCR assay specific for its corresponding alternatively spliced variant (for example, HMGCR13 template with 13 qPCR assay).
Firstly, determining which transcript of a gene to analyze is controversial.
EnsemblCompara [ 2], for example, selects the longest transcript of a gene.
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We also detected transcripts of a gene similar to the hcrL gene encoding 4-hydroxybenzoate-CoA ligase, however, it is not possible to differentiate between hcrL and the benzoate-CoA ligase gene (bzlA) (Table 1).
Analyses of these data focused primarily measuring the relative abundance of transcripts of a gene, while treating most or all transcript isoforms as equivalent.
In situ hybridisation mostly detects the transcripts of a gene.
Accurately annotating all transcripts of a gene is crucial.
This gene representation lets us easily enumerate all possible transcripts of a gene.
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