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In brief, reverse transcription of each mRNA transcript occurred during incubation for 5 minute at 70°C, followed by incubation for 1 hour at 42°C and finally at 70°C for 10 minute.
The lowest point of noa transcript occurred on day 2 with a 71% decrease compared to ds-egfp control(Fig. 3A).
These indicate that each of these 120 transcript occurred in a specific subtraction.
Addition of the spliced leader sequence to each transcript occurred at the same sites as used in T. brucei (Fig. 2D).
Alternative splicing of this transcript occurred to utilize an alternative acceptor site (CAG) 80 nt downstream of functional acceptor site for intron 7, which introduced a deletion of 27 amino acid and a frameshift that leads to an in-frame stop codon.
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Preferential accumulation of the loop mRNA, however, indicates that degradation of the hairpin transcript occurs mainly via processing of the stem of the hairpin.
These findings suggest that the foraging frequency-dependent increase in the kakusei transcript occurs without an increase in the number of kakusei-positive neurons.
Mistranslations of the transcript occur, rescuing the mutant and restoring the wild-type function.
Polyadenylation of the transcript occurs at the polyadenylation signal (AATAAA) contained with the B2 sequence.
Internal translation of the Cx43 transcript occurs but is not cap-independent and requires translation upstream of the internal start codon.
Finally, co-transcription of nad6 with an unknown ORF led to the generation of a large transcript occurring in a CMS lineage of Mimulus guttatus [ 9].
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