The cumulative read depth along each transcript was calculated to examine the rate of transcription along transcript models.
This involved building transcript models supported by transcriptional or translational evidence, primarily in the form of messenger RNA (mRNA), expressed sequence tag (EST) or protein homologies.
In total, 56 transcript models were tested by reverse transcription polymerase chain reaction (RT-PCR) in independent An.
All non-redundant, multi-exon alignments of transcriptional evidence at an individual locus are used to build transcript models.
Current studies using the ascidian embryo benefit from extensively annotated genomic resources to characterize transcript models in silico.
All variations were mapped to all transcript models, which led to multiple annotations for several loci.
Consequently, using just 1-kbp genomic regions as done previously [14], likely predict poor transcript models with high frequency.
In accord, we found, while predicting the transcript models of the previously non-annotated FBX genes, a substantial number of putative pseudogenes containing frameshifts or premature stop codons.
Transcript models for An.
Furthermore, several of the predicted transcript models were experimentally validated.
Approximately 87%% of reads matched known soybean transcript models.
