We give further evidence that the genomic BCR-ABL amplification results in an increased level of BCR-ABL transcript linking two potent mechanisms of resistance against imatinib treatment.
The apparent contrast between the enhancement of digestive activity, which is centered in the midgut, and the decrease in transcripts linked to transcription and translation may reflect changes in transcript abundance occurring at the whole-body level.
The high expression level of ribosomal 16S, an essential gene for the translation of mitochondrial messenger RNAs into proteins, and the presence of some transcripts linked to transcription (BRbwC19 and 20, Table 2) is then consistent with a high protein turnover in this tissue.
Carbohydrate utilization profiles (Figure 3a) were dominated by transcripts linked to central carbohydrate and glycogen metabolism subsystems.
We sought to determine whether v-Abl kinase activity could mimick IL-3 in controlling the rate of decay of transcripts linked to myeloblast growth and survival.
We therefore determined whether IL-3 altered the turnover of a number of transcripts linked to myeloblast growth or function, in addition to p21.
It has previously been shown in a study of co-regulation in a mouse F2 intercross [20] that groups of highly correlated transcripts linked to the same genomic location can be identified in a functionally informed genome-wide correlation analysis.
If IL-3 coordinates this phenotype in part through stabilizing mRNAs, then a subset of transcripts linked to these functions will decay slower in IL-3 than when IL-3 is withdrawn or replaced with the differentiating cytokine G-CSF.
The presence of many transcripts linked to cellular proliferation and transformation in RPCs most likely reflects the dynamic nature of the control of cell division in a developing tissue.
Interestingly, we also observed down-regulation of several transcripts linked to phagocytosis.
