Transcription rate and transcript length drive formation of chromosomal interaction domain boundaries.
Total transcript length in number of codons was also considered.
Linear plots for Figure 2. RNA SPOTs has little bias in GC content and transcript length.
Having demonstrated that transcript length and AA AG ratio regulate protein levels in an Elongator-dependent manner, we next investigated the identity of specific Elongator targets.
In order to identify high confident completely novel transcripts for potential experimental validation, we applied three simultaneous filters namely phastCons score, expression and transcript length.
Novelty score of a transcript is defined as the percentage of non-overlapping novel transcript length to the reference annotated transcriptome (Fig. 2b).
Figure 3c shows that the ability to maintain normal expression levels becomes increasingly dependent on Elongator as AA-bias and transcript length increase.
In total, 39.4 Mb bases are assembled and the average transcript length is 1,269 bp.
This value could measure the transcripts abundance with transcript length, thus we can compare it among different genes or samples.
The coding sequence of galectin-4 is specified by nine exons and the transcript length is 1 kb.
The transcript length is predicted to 4.56 kb excluding the polyA tail (Figure 3A).
