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An alternative method of ensuring transcriptional consistency focuses on removing unwanted elements from the mRNA transcript itself, after transcription but before translation.
Different genetic strategies have yielded specific lessons about the roles of lncRNA transcription, the lncRNA transcript itself, and underlying sequence elements.
We find that CUTs and SUTs are predominantly retained and degraded in the nucleus, suggesting that their functions primarily arise from the act of transcription rather than the transcript itself.
One remaining difficulty is selecting the manner by which to disrupt lncRNA transcription in a mouse model without prejudicing the outcome of whether the transcript itself, the act of transcription, or even both, transact function (Hainer et al. 2011).
One major difficulty in assigning function to ncRNAs is to distinguish between the effects of transcription per se and a function of the transcript itself.
It has recently been demonstrated that it is the transcriptional overlap of the Airn ncRNA and not the ncRNA transcript itself that induces the imprinting of Igf2r[ 72].
Similar(27)
We considered transcripts, which exhibited expression levels correlated with meat quality traits, and which had eQTL on the same chromosome as the transcripts itself.
That way, other sub-themes that arose in the transcripts itself could also be added to form the final coding template [ 30, 31].
One of the government's petty and vindictive tactics has been its refusal to release transcripts of the hearings to the media or the broader public (even as it generates a transcript for itself).
Accordingly, induction of the HIF-1-regulated VEGF was detected in bacterial infections with S. aureus, P. aeruginosa and E. coli by VEGF mRNA induction whereas HIF-1α mRNA transcript levels itself again appeared unaffected (Fig. S2).
HIF-1α mRNA transcript levels itself were unaffected (Fig. 4D) excluding direct effects of S. aureus on HIF-1 transcription but suggesting oxygen-dependent posttranslational mechanisms of HIF-1 activation.
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