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IP of mRNA/YB-1 protein complexes revealed a five-fold enrichment of YB-1 on the TGF-β1 transcript in comparison to the β-actin (Figure 9D).
In addition, provision of LDL-derived cholesterol to HepG2 cells incubated with 0.5 µM simvastatin (Figure 1C) resulted in 10 17% (12% average) greater suppression of the HMGCR13 transcript in comparison to the HMGCR13 transcript (p = 0.049), suggesting that sterol loading induced HMGCR alternative splicing.
Inhibition of WISP3 expression in HME cells resulted in increased expression of RhoC transcript in comparison with HME cells transfected with the control empty vector (Fig. 1).
The results of Northern blot consistently reported that Nonabokra plants have 4 5 times higher level of OSBZ8 transcript in comparison to M-1-48.
Relative quantification of the target gene transcript in comparison with β-actin (housekeeping gene) expression levels in the same sample followed the ΔΔCt method (Livak and Schmittgen 2001).
Read-abundance validated reads are based upon sequences predicted to be found in the mutated transcript in comparison with sequences that are expected to be excised from the mature transcript in the absence of a mutation.
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However, in contrast with this paucity of protein coding 'genes', there is an enormous complexity in transcription and the protein coding mRNAs contribute to a very small fraction of transcripts in comparison with the different varieties of non-coding RNAs (ncRNAs).
Landrace 85-2 showed a poor response in the number of up-regulated transcripts in comparison to the other landraces.
Inhibition of Erk phosphorylation by the Mek1 inhibitor U0126 partially blocked v-Abl stabilization of transcripts in comparison with imatinib (Figure 4E).
Aberrant expression of miRNAs in cancer is characterized by abnormal expression levels of mature or precursor miRNA transcripts in comparison with those in the corresponding normal tissues.
Moreover, we did not find any dramatic difference in the nuclear vs. cytoplasmic ratio of Pdcd2 transcripts in comparison to control genes.
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