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IsoEM is an expectation-maximization algorithm for transcript frequency estimation.
The prediction accuracy is positively correlated with the predicted minor transcript frequency.
A sharp decline of the transcript frequency is displayed at a distance >3 kb.
For the EST frequency to represent the absolute transcript frequency, sequencing efforts must be large and sampling must be unbiased.
A key advantage of pyrosequencing is that it provides estimates of the relative transcript frequency for each KIR.
fExpression profile means transcript frequency per million calculated from the number of ESTs in a certain library.
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Lactation stage specific mammary transcript frequencies were evaluated from the distribution of ESTs within each stage specific library.
It overcomes the problem of reads mapping to multiple transcripts using iterative framework which simultaneously estimates transcript frequencies and imputes the missing origin of the reads.
The Gli-A2 transcript frequencies that were obtained by pyrosequencing are shown in Figure 6 for two individual plants per accession.
Other ULs with high transcript frequencies in spores of G. clavigera encoded proteins involved in energy metabolism and protein biosynthesis, which is consistent with respiration and protein biosynthesis activated early in germination [ 30].
SAGE and DNA microarray generate large libraries of mRNA sequences enabling researchers to generate differential gene expression lists in disease states by statistical comparison of transcript frequencies between two or more conditions.
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