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Notably, the alternative splice sites did not affect the reading frame of the transcript except for Δ498 1340/Δ1655 1748.
GeneBank accession numbers are indicated for each transcript except for variant V where transcript ID from Vertebrate Genome Annotation (VEGA) database is shown.
The sequence we identified (GU936962) was very similar to the NCBI transcript except for one amino acid change located in the second extracellular loop.
Using RT PCR with a set of overlapping primers located within the region from MBS10 to miR-379, we were able to detect a continuous transcript except for a small gap.
These data indicated that the FOXO1A-2 and FOXO1A-3 cDNAs were derived from transcripts that were identical to the canonical transcript except for the alternative splicing within the 3'-UTR.
Gene specific primers that encompass one intron were defined for each transcript (except for HOXA9 gene) using the program Primer Express version 2. To design intron-spanning primers, putative intron-exon boundaries were deduced from the Genome Browser Database (http://genome.ucsc.edu/cgibin/hgGateway?hgsid=105081852&clade=vertebrate&org=Cow&db=0).
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All detected transcripts were found to result from independent transcription, except for Transcript B, which was linked to the transcription of the downstream gene rpsB (BP1419), encoding the 30S ribosomal protein S2.
Tomato photoreceptor transcripts, except for CRY1b, appeared to be synchronized and peaked during the presumptive afternoon, (Figure 4A-D).
Interestingly, leaves infected with the avirulent pathogen accumulated higher and diverse levels of all CHS gene transcripts except for CHS4.
In this respect, we observed that all transcripts, except for UBD, were altered after TSA treatment of fibroblasts (Fig. 11).
Each of these transcripts, except for Mucin 1 (Muc1), was expressed at much lower levels in H2BGFP+/CD24+/CD29lo cells.
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