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Using the Ensembl annotation pipeline12 and RNA-seq transcript evidence, we predicted a total of 18,872 protein-coding genes.
We predicted the protein-coding genes in BMR using a combination of homology-based and de novo methods, as well as transcript evidence.
To investigate this aspect of the water-to-land transition, we identified 684 genes (657 genes possess transcript evidence; see Supplementary Note 5) that are present in mudskippers but not in other analyzed teleosts.
RNA sequencing de novo assemblies for A. hypogaea and the diploid Arachis species were supplied as transcript evidence along with available EST and mRNA data sets from NCBI for these same species.
It corresponds to Swissprot entry Q59FL9, for which only transcript evidence is available.
There is no transcript evidence for these genomic sequences (mouse Cldn4-like and rat Cldn13-like) indicating that they are unlikely to be transcribed.
Our study found 3324 candidate STEPs lying in motif sequences of 5' non-coding introns and further mining revealed 170 with transcript evidence of intron retention.
Weight of transcript evidence used for gene prediction via EVM.
These have full-length transcript evidence and lack ORFs.
The majority of OGSv3.2 genes were supported by transcript evidence.
Individual gene pairs were examined for transcript evidence at the orthologous locations in cow.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com