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Up arrow, up-regulated transcript; down arrow, down-regulated transcript.
The membrane intrinsic protein encoding transcript down regulated at 1 d.p.i., was also found to be down regulated at 30 d.pi.
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For example, in the case of global transcript level changes in cells with inhibited transcription, one might expect to detect predominantly transcript down-regulation, whereas after applying quantile normalization it is very probable that a significant number of up-regulated transcripts will be observed, due to intensity distribution transformations.
Because SMILE transcript down-regulation decreases transcripts involved in protein degradation, we tested whether SMILE was involved in proteolysis.
The shape of the running sum curve shows a sharp peak of the miR-145 7mer seed sites for the most down-regulated genes suggesting that target transcript down-regulation to a large part is due to direct effects of miR-145 targeting.
Cluster profiles 1-4 as well as 12 all describe different patterns of transcript down-regulation.
Indeed, in Atlantic cod exposed to 46% hypoxia for six weeks we observed CYP1A transcript down-regulation [ 39].
VLR-induced transcript down-regulation includes pathways involved in fundamental macrophage functions such as inflammation, chemotaxis, metal and cation transport.
As both members of these sense and antisense transcript pairs appear to be abundant it seems unlikely that their role is sense transcript down-regulation.
Real-time PCR was used to accurately quantify the level of ERβ transcript down-regulation induced by DHT and BPA treatment of LNCaP prostate cancer cells.
Our primary hypothesis in this instance was that if biological off-target activity of either siRNA occurred via a miRNA-like transcript down-regulation mechanism then it should be possible to detect enrichment of putative off-target transcripts (i.e. using enrichment of the complementarity seed motif as a proxy of transcript down-regulation), as described for the hsa-miR-124 dataset.
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