GMP participated in the design of the study, development and pre-testing of the data collection instruments, training of the research assistants, conducting the interviews, checking and validation of the transcripts, data analysis, and drafting of this manuscript.
In this review of a large number of CRC transcriptional studies, we identify recurrent sources of technical variability that encompass collection, preservation and storage of malignant tissue, nucleic acid extraction, methods to quantitate RNA transcripts and data analysis pipelines.
This filtering process resulted in 3,044 (out of 47,231) transcripts for data analysis.
These field notes will be used in conjunction with interview transcripts during data analysis.
FO participated in the construction of the two MDM SAGE libraries, the RT-PCR validation of human transcripts and data analysis.
Transcript quantification and data analysis were as above.
In line with the transcript data, western blot analysis did not show significant differences in Bcl-2, Mcl-1, XIAP and Livin protein expression with respect to TTL-subgroups.
VAE supervised the project, read all transcripts and supported data analysis and helped draft the manuscript.
Finally content analysis was performed on coded transcripts using qualitative data analysis software (NVivo 9, QSR International Pty Ltd., Australia).
The preliminary coding framework of index codes was agreed and applied to transcripts using qualitative data analysis software Atlas Ti [ 32]. 4. Charting.
