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The relative transcript copy numbers demonstrated that the transcription of rhA1AT and dhfr genes were correlated due to the IRES linkage, although the results also suggested that the protein expression were not solely dependent on transcript levels.
However, in highly expressed genes, n is unlikely to be much smaller than k; thus, both gene length (L) and transcript copy number (n) contribute to the duration of transcription.
Standard curves have been constructed for all assays, thus allowing for absolute quantification of mRNA transcript copy number.
Based on a diel expression study, the rbcS transcript copy number per cell ranged from 2.16 × 104 to 5.35 × 104, with the highest expression during early to mid photoperiod.
We also compared six of these genes to their orthologs in a second genetically-isolated strain of C. uncinata to reveal that patterns of nanochromosome and transcript copy numbers are conserved between strains.
The contract stipulated that a transcriber was to be provided and paid for, with the only transcript copy provided solely to Clinton herself.
We know the transcripts exist - Clinton herself required a transcript to be made and paid for by each speech sponsor - and then required that the only transcript copy be turned over to her.
Using ΔCt analysis, normalized data were used to estimate the transcript copy number in infected mosquitoes.
Transcript copy numbers for FcγRIIb and beta-actin were quantified by comparing to a standard curve generated from serial log-dilutions of the target DNA [44], [45].
Values were expressed as transcript copy number per 50 ng of total input RNA, which were determined in each sample by interpolation with the respective standard curves.
Actual transcript copy number was determined during the qPCR assay using the Applied Biosystems Sequence Detection Software and a standard dilution curve to establish a linear relationship between CT value and copy number of each reaction.
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