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Our data shows that the genes in the irradiated cells can be categorized into three different groups with regard to the changes in RNA transcript content: 1) genes with an increased level of RNA transcripts, 2) genes with a reduced level of RNA transcripts, and 3) genes that remain with the same RNA transcript level when compared to the non-irradiated control cells (Figure 2, a).
This was repeated several times using the transcript content, initial notes and themes.
However, some discrepancies have been found between transcript content analysis and proteins content analysis.
Additional file 2: Standard curves used for the measurement of IGF1 gene transcript content in PBMC.
This suggests that the AtRAD21.1 transcript content increase, induced by DNA dsb, may be required for DNA dsb damage repair.
A coding scheme was developed based on issues that emerged while reviewing transcript content and a priori topics of interest.
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The transcript contents were quantitatively determined using each purified cDNA clone as a calibration standard.
a - c Transcript contents of the OsAS1 (a), OsAS2 (b) and a control Actin1 (c) in the basal portions of shoots of wild-type plants (WT: black column) and gs1 2 mutants (gs1 2: grey column) at the seventh-leaf stage for seedlings grown in water for 3 d followed by treatments with (+NH4+) or without (-N) 1 mM NH4Cl for 8 h.
Two researchers (KL, JL) conducted an independent analysis of the transcript contents.
We found a statistically significant difference in the annotated transcript contents between the two datasets, consistent with the idea that large non-transcribed regions have distinctive chromatin structures.
The mRNA transcript contents of samples FS-6 and FS-40 were clearly distinct (figure 2B).
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