In vitro transcription using RNAP yields a transcript containing the target RNA fused to the glmS ribozyme and the binding sequence.
There is convincing experimental evidence that mutagenesis occurs through reverse transcription of a cDNA from a transcript containing the TR [ 3, 7].
Pol II is recruited to the HIV promoter and initiates transcription, which stalls after a 30 50 nucleotide transcript containing the trans-activating response region (TAR) is formed.
The AGO complex is guided by the recruited miRNA to bind onto a specific target transcript containing a region highly complementary to the miRNA.
CML cells have two novel products: an 8.5-kilobase RNA transcript containing both abl and bcr and a 210-kilodalton phosphoprotein (P210) recognized by v-abl-specific antisera.
In all cell lines, the transcript containing the first coding exon (exon 1; Figure 6A) was expressed at similar levels as the transcript containing the exon -2a/exon -1 boundary, which is the most abundantly expressed -2 exon (Figure 6C).
The levels of all the different p110δ transcripts are higher in leukocytes compared to non-leukocytes, with the p110δ transcript containing exon -2a most abundantly expressed.
Because the transcripts of UTP14C and GT8 overlap the possibility of a single transcript containing open reading frames from both genes arises.
However, the transcript containing miR-200c extended 5' of the stem-loop, as determined by PCR and sequencing (Figure 5D and Table S7).
All cell lines tested (except CT26 colon carcinoma) expressed at least one type of transcript containing a -2 exon (Figure 5B).
Confocal microscopy revealed that the SHMT protein expressed from the SHMT2 transcript containing exon 1 (referred to as SHMT2-YFP) and CFP-mito protein co-localized to mitochondria.
