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As well as comparing human and mouse RefSeq collections, we also compared the RefSeq transcript collection to the transcripts included in ASPicDB [ 13], which collects all putative alternative isoforms for a given gene determined by the ASPIC algorithm [ 28, 29].
The influence of transcript collection and gene definition methods was tested using two microarray data sets.
In the genome based transcript collection, of the 59 possible genes, eleven were missed.
In contrast, the JGI Besttranscriptpt" collection has the opposite ratio (~20/80 GC/GT).
The JGI Besttranscriptpt" collection is composed of 68% ab initio predicted genes [ 8].
Our full-length cDNA library reported here is the first large scale transcript collection for P. striiformis.
Similar(34)
Comparison to publicly available transcript collections suggests that the assembly covers ~75-80 ~75-80e complete transcriptome.
Number of Illumina HiSeq sequencing reads from each developmental stage mapped to selected reference mRNA transcript collections.
This can be explained if these transcript collections are not completely normalized or if there is bias for certain sequence motifs within gene transcripts.
We tested the TSS predictions in the gene and transcript collections of EnsEMBL55, CAGE and DBTSS based on our experimentally verified human RP promoter set (Additional file 3).
Moreover, RefSeq transcript collections (and gene annotations), usually employed to establish and assess orthology relationships, appear to be highly incomplete with respect to alternative splicing.
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