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The class transcription represents the production (relationship produced by) of a primary transcript (class primary transcript) from the information codified (relationship has participant) by a gene (class gene).
To examine the distribution of hits for a particular protein across all members of a transcript class (e.g., Cbc1 hits in mRNAs), we used two related approaches, both performed on the most abundantly bound members of the transcript class.
Long non-protein-coding RNAs (lncRNAs) are proposed to be the largest transcript class in the mouse and human transcriptomes.
The 2-fold upregulation of the seventh sHSP transcript (class I) is comparable to the upregulation of the associated protein.
A breakdown of hits by transcript class revealed a broad range of substrate specificities for the tested proteins.
The merged assembly transcripts provide a uniform basis for calculating transcript expression in each condition and the transcript class code.
Similar(50)
The last exon (exon 9) is the main protein-coding exon, and several distinct transcript classes are formed when transcription is initiated at various other exons [Pruunsild et al., 2007].
Contrary to the expectation that antisense transcription or processing regulates alternative polyadenylation of the sense gene, we find that the two alternative sense transcript classes are independent of antisense transcription.
We also investigated the transcript structure of different transcript classes by comparing the number of exons and length distributions.
We observed that, all three transcript classes exhibited significantly different expression profiles for each developmental stage (Wilcoxon rank sum test, p-value < 0.001, See Table S4), with known and PNTs exhibiting significantly higher expression compared to CNTs.
We found each pair of these transcript classes to be significantly different in their extent of conservation (p < 2.2e-16, Wilcoxon rank sum test) with median conservation scores 0.67, 0.76, and 0.13 for known, partially novel and completely novel transcript groups respectively.
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