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Northern blot analysis disclosed various transcript characteristics, such as expression level, size and the presence of isoform.
To investigate transcript characteristics in the genes most affected, we compared transcript lengths (taken as the median cDNA length for each gene) between the different groups.
The genes were initially identified by in silico analysis and their transcript characteristics, region-specific expression, postnatal expression, and hormonal regulation, and characteristics of the expressed proteins were characterized in vitro.
In order to aid the assessment of post-experimental RNA degradation, more complex methods have been developed including RNA degradation plots [ 62] or mixed effect models based on individual probe and transcript characteristics [ 63].
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Furthermore, rps10b-1 did not affect levels of CCD7 or CCD8 transcript characteristic of the MAX2- or max2-1-backgrounds (Additional file 3: Figure S2).
All four COLIGOs generated the large transcripts characteristic of RCT.
The spliced transcripts characteristic for polysomal RNA, were not detected in the total RNA fraction, which suggests that transcripts that are actually translated represent only a small subgroup of the mRNA population.
Similar to the wild type mice retinal explants, a significant increase in BrdU+GS+ cells was observed in Jag1+Wnt3a-treated rd mice retinal explants, compared to controls, accompanied by an increase in the expression of transcripts corresponding to Ki67, CyclinD1, Hes1, Hes5, and Lef1, and an increase in SP cells numbers with transcripts characteristic of proliferating neural progenitors (Fig. S2).
However, Dicer-null NS cells fail to complete a differentiation program, and in the case of gliogenesis this appears to be the result of not up-regulating expression of a large set of cell-specific transcripts characteristic of the differentiated astrocyte.
We found that the fruits of mycorrhizal plants show regulation of transcripts characteristic of climacteric fleshy fruits and a core set of genes typically modulated by symbiosis.
These data point to specific downregulated transcripts of cells resident to the wound as well as upregulated transcripts characteristic of infiltrating leukocytes that could be useful markers of poorly healing wounds and indicators of wound-specific treatments for improving outcomes.
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