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Relative expression levels were normalized using σA as an invariant transcript, and data was analyzed using the ΔΔCt method as previously described [30].
The thorough process of focus group transcript and data triangulation ensured robust qualitative data analysis.
Genes were considered not differentially expressed if Cuffdiff returned an "OK" value for the test status (at least ten reads aligned to the transcript, and data were sufficient for testing for differential expression) but the expression levels were not significantly different.
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In this review of a large number of CRC transcriptional studies, we identify recurrent sources of technical variability that encompass collection, preservation and storage of malignant tissue, nucleic acid extraction, methods to quantitate RNA transcripts and data analysis pipelines.
The Pentagon released transcripts and data from an airstrike that resulted in the killing of 23 Afghan villagers on February 21 , 2010 in Uruzgan Province after a drone crew mistook them for Taliban militants.
The same analysis framework will be followed with all transcripts and data will be prepared for coding, including reading of all transcripts and familiarisation with data.
FO participated in the construction of the two MDM SAGE libraries, the RT-PCR validation of human transcripts and data analysis.
At random intervals throughout the study, a sample of recordings was independently reviewed and compared with transcripts and data collection forms by the CAPRISA study coordinator, a native Zulu speaker.
The research staff who performed the coding met with the principal investigator to confirm the coding scheme to be used across all three focus group interview transcripts, and data with common meanings were coded identically through comparative analysis.
For comparison of gene expression with metabolite data, transcript and metabolite data were z-transformed prior to pairwise correlation (Pearson correlation coefficient) analysis.
Much of this data is already out there -- unemployment insurance, wage records, transcript and program data, job openings data, and detailed information on occupational competencies have already been compiled.
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