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In our study we observed in Li 2 mutant fibers higher transcript activities of genes related to replication as well as induction of ROS responding genes.
Further functional profiling by metagenomics, metatranscriptomics and additional modalities will thus be required to understand how and why microbial genes and genome compositions, pathway and transcript activities, and metabolic processes are altered in inflammatory conditions, health and disease.
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Ethephon elicited greater transcript activity from genes implicated in defence against fungal and insect attack and oxidative stress (Table 2).
It is essential to also include knowledge about pathway-specific transcript activity.
Recent proteomics profiling (e.g. [ 56]) will allow the consideration of protein activity rather than transcript activity.
Higher expression of ethylene biosynthetic genes correlates with higher transcript activity of glutamine synthetase in Li 2 mutant fibers.
As a result it fails to reproduce the qualitative behaviour of transcript activity in a negatively auto-regulated gene.
For the –443 locus, the authors suggested that –443 T had a lower promoter activity that could regulate the OPN gene transcript activity, thus influencing OPN expression.
In the absence of distinguished differences in the amount of mRNA, transcript activity still may be altered or posttranslational mechanisms may affect protein structure, level, or cellular distribution.
These studies provide information on the mechanism under which the -443T/C polymorphism regulates the OPN gene transcript activity, thus influencing OPN expression.
Common themes in gut microbial ecology have emerged among these diverse diseases, but they have not yet been linked to targetable mechanisms such as microbial gene and genome composition, pathway and transcript activity, and metabolism.
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