Exact(5)
Another metric of assembly performance is transcript accuracy.
Evaluation of transcript accuracy was initially based on mapping useful reads of two randomly selected libraries of each sole transcriptome using Bowtie2 (not shown).
For example, the human ENCODE Genome Annotation Assessment Project (8) has shown that the average multiple transcript accuracy (e.g. the accuracy in predicting all isoforms of a gene correctly) of tested prediction tools reached only 40 50%.
To gain a general overview of transcript accuracy, a custom EST library, constructed using the recurrent parent Chinese Spring (CS) containing 51,449 Sangerangingnced ESTs ranging from 100 bp to 922 bp in length (N50 of 634 bp) was used (Zhao GY et al., unpublished).
Recent publications also showed that Trinity had a consistently better performance than the other assemblers as SOAPdenovo, ABySS, trans-ABySS, Oases with single K-mer value, when measured by transcript accuracy, integrity and completeness, and sensitivity to assemble transcripts from low to high expression levels [ 30].
Similar(55)
Two bilingual, bicultural researchers used a three-phase transcription and translation process to ensure transcripts' accuracy.
The expression is evaluated in three different measures: transcript expression accuracy (Theta), transcript within-gene relative proportion accuracy (WGE-True) and inter-replicate consistency (WGE-Inter).
I transcribed each interview verbatim and reviewed each transcript for accuracy.
Minor revisions and additions have been made to the initially published version of the transcript for accuracy and thoroughness.
The primary researcher (CB) attended all focus groups and reviewed each transcript for accuracy.
A bilingual researcher reviewed each transcript for accuracy of speakers' actual words and accurate translation from Japanese to English.
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