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Cas9 mRNA was transcribed with the mMESSAGE mMACHINE® T3 Transcription Kit (AM1348, Ambion).

All of the interviews were recorded and transcribed with the participants' prior consent.

All interviews were recorded and transcribed with the interviewee's consent.

The audio log files were then retrieved and transcribed with the due omissions.

The RNA was reverse transcribed with the Script cDNA synthesis kit (TaKaRa).

Interviews were recorded and transcribed with the help of the same research assistant to minimize translation errors and clarify information.

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In L. bulgaricus, previous study revealed that the pyk gene is co-transcribed with the gene encoding phosphofructokinase (Pfk), and these two genes constitute the pfk-pyk operon16.

Total RNA was isolated with RNeasy kit (Qiagen), quantified and 1 μg of RNA was reverse-transcribed with the Superscript II reagent (Invitrogen).

Total RNA was reverse-transcribed with the Superscript III first strand RT-PCR kit (Invitrogen).

Equivalent amounts of RNA were reverse-transcribed with the iScript cDNA Synthesis Kit (Bio-rad Laboratories; Hercules, CA).

It has been shown that some intronic miRNAs are co-transcribed with the gene that contains them.

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