Sentence examples for transcribed template from inspiring English sources

For example, formation of G4 structures in the non-transcribed strand could facilitate high transcription rates by sequestering the transcribed template to prevent re-annealing to the G4-rich complementary strand.

Real time PCR was done using 5 PRIME SYBR mastermix for Real-time PCR (5 PRIME, Maryland, USA) in an Eppendorf Mastercycler using 2 μl of diluted reverse transcribed templates.

Here, we describe the identification in hyperthermophilic Crenarchaeota and a Korarchaeon of an orthologue of the eukaryotic transcription elongation factor Elf1, which has been shown to function in chromatin structure maintenance of actively transcribed templates.

We found that propagation of the repeats on plasmids was overall normal, but that transcription of the repeats in either orientation with either AAT or ATT serving as transcribing template induced significant changes in colony morphology, leading to formation of convex colonies from normal colonies, which then progressively transformed into transparent colonies.

We employed an ultrastable optical-trapping assay to follow the motion of individual molecules of RNAP transcribing templates engineered with repeated sequences carrying imbedded, sequence-specific pause sites of known regulatory function.

Unmodified RNA substrates were transcribed from template DNA using T7 RNA polymerase and purified by 6 to 20% denaturing PAGE.

The subpathway transcription-coupled NER (TC-NER) removes damage from actively transcribed DNA templates.

Additionally, Hes5-1 anDelta1a1 [ 4] probes were also transcribed from templates generated by PCR and containing the respective coding regions.

This observation suggests that MGL RT reverse-transcribes the template RNA until the A/U nucleotide of the motif, where it can jump to another RNA before the GCC triplet.

Synthetic mRNA was transcribed from the template plasmids using Message Machine T7 or (T7 Ultra kit for ARCA-capped mRNA) (Ambion).

Pause density, ρpause, is defined as the average number of pauses per bp of template transcribed.