Sentence examples for transcribed products from inspiring English sources

Since alternatively spliced or transcribed products encoded by the same gene have different biochemical properties [ 11], we assigned different IDs to each bZIP TF and its transcript variant, as reflected in PPI and TF_TARGET tables.

In order to confirm the differential expression of these genes, reverse slot blot hybridizations were performed: DNA from four to ten clones obtained from each differentially displayed band was fixed on a membrane and hybridized with the corresponding reverse transcribed products from either BTH or H2O treated plants (Fig. 4).

A standardized mixture of internal standard (SMIS) competitive templates is added to the reverse transcribed products prior to PCR.

PCR amplification of reverse transcribed products was carried out in 25 μl reactions, containing 200 nM primers, 100 250 ng cDNA, 350 μM dNTPs, 2.25 mM MgCl2 and 1.25U Taq Extensor Hi-fidelity DNA polymerase (ABgene).

The transcribed products are stored (usually) in inactive forms in multi-compartmented dynamic steady states (one for each gene product) as a component of the routine metabolic activity of the cell.

Transcripts unique to diapause in Kmar proved to constitute only a miniscule fraction (0.1 %) of the total pool of transcribed products.

One microliter (1 ng) of reverse transcribed product was amplified with TaqMan® Universal PCR Master mix (Applied Biosystems) and gene-specific primers designed by Applied Biosystems (Flt3 Mm00438996_m1 and GAPDH 4308313).

One µL of the reverse transcribed product were amplified in a temperature gene cycler (Gene Amp PCR System 9700; Applied Biosystems, Foster city, CA, USA) using 1 nmol of each sense and antisense primers and 1 U of Platinum Taq DNA polymerase (Invitrogen Life Technologies, San Diego, CA, USA) in a final volume of 50 µL.

The reverse transcribed product corresponding to 0.1  μg of initial RNA was subjected to PCR amplification in a thermal cycler.

PCR of the reverse transcribed product was done using gene (FAT gene) specific primer pair (Forward TTCAAAATAggTgAAgAgACAggTggTg 3' & reverse 5' TTgTgATgAgACCTgTTTTAggATg 3') in PTC 200 thermal cycler (MJ Research).

The reverse transcribed product was purified on denaturing PAGE, and amplified by PCR using prTempCCC- and prSeq (5′-CCTTGTCGTCACCTCCCA-3′) as primers.