In vitro transcribed, PAGE-purified, refolded RNAs (50 nM) were incubated with 0.5 μM IRE1α-KR43 for the indicated times in RNA cleavage buffer (20 mM HEPES pH 7.5, 70 mM NaCl, 2 mM Mg OAc 2, 1 mM TCEP, and 5% glycerol).
In this book, we discover a little boy who grew up with 'patriotic newsreels, suspicious of all British adults', a nine-year-old steeped in GA Henty, Dickens and Charles Kingsley, who transcribes pages of Westward Ho!, and for whom 'home' was the England of AA Milne, Just William and Chums.
In 1933, a copyist transcribed twenty-three pages of the score, and at a later date Sibelius's publisher may have bound the manuscript in a set of seven volumes.
But when his memory was still sharp, his daughter Dorothy transcribed 48 pages of his taped recollections, which became the basis of a four-minute first-person narrative produced by the museum.
The interviews were digitally recorded (4 hours 41 minutes in total), transcribed (167 pages in total), and anonymised.
As I transcribe those pages from my notebook into my computer I have many moments of despair -- because I can see how little of it I'm going to use.
Between hanging at the bar, perusing the exhibition, sharing Rock & Roll stories, making new friends and enjoying Weiland videos on the big screen, many even took the time to transcribe eight pages of lyrics from their brothers and sisters from across the country and around the world.
Members of the committee, with the benefit of 150 hours of depositions transcribed into 4,000 pages of testimony, focused on particular elements in Mr. Verniero's accounting.
