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The cDNA was then transcribed back into cRNA, using T7 RNA polymerase, labeled with either Cy3 or Cy5 dye.
The RTEs proliferate through an RNA stage that is subsequently reverse transcribed back to genomic DNA [ 1].
If an RNA intermediate is involved, there must be a point at which the transcript is reverse transcribed back into cDNA.
Transcription and Reverse Transcription: As our starting clonal sample is DNA, we first transcribe our DNA to RNA so that it can be reverse transcribed back to cDNA, enabling us to evaluate errors that impact upon the RNA template.
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Some of the researchers are already at work on some applications; Hain's award-winning team is collaborating with the BBC to transcribe its back catalog of audio and video footage.
Bach wrote this canon as one of 14 that he transcribed on the back of his copy of the Goldberg Variations, Gardiner also tells us, and in his submission for admission to Mizler's Society of Musical Sciences in 1747, the work was included along with the first Hausmann portrait (the musical riddle is in that picture too), as well as his Canonic Variations on "Von Himmel hoch".
All sessions were recorded, transcribed, translated, and back translated to ensure accuracy and quality.
Translations were performed by bilingual translators and back transcribed to ensure accuracy.
Retrotransposition occurs when the messenger RNA (mRNA) from a gene is reverse transcribed and inserted back into a random position in the genome (Hollis et al. 1982; Karin and Richards 1982; Ueda et al. 1982).
The interviews were recorded on audio cassettes and subsequently transcribed, translated and back-translated.
The recordings are transcribed, annotated, and fed back in hopes of improving Alexa, the software that powers Echo devices.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com