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Elongation is halted when the enzyme transcribed against a downstream torque load of 11.0 ± 3.7 pN nm.
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In the absence of TFIIS, Pol II was only able to transcribe against an opposing load of 7.5 ± 2.0 pN, because its propensity to backtrack stalled the enzyme at high opposing loads.
All forms were transcribed, checked against the originals, fully anonymised and printed in a standard format.
Second, weekly CHW summary logbooks were transcribed and verified against individual CHW visit reports to create an electronic database of CHW home visits.
Focus group recordings were transcribed and verified against the original recordings.
All focus group discussions were recorded on audio tape, transcribed, and verified against the tapes.
The interviews and focus groups were transcribed, transcription checked against recordings, and then analysed thematically.
Step 1: As data were obtained, interviews were transcribed and rechecked against the audiotapes, then translated to English and verified.
The interviews were transcribed verbatim, validated against the recording, and the data stored in a password protected location.
Together these results suggest that Srs2p has a particularly important role in protecting the highly transcribed rDNA locus against Rad51p-dependent hybrid formation and repeat instability.
The questionnaire was piloted using cognitive interviewing methodology, interviews were audio-recorded, independently transcribed, content checked against original recording, offered to participants to review, and then analyzed using key response patterns and themes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com