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The back wall is a huge chalkboard usually covered in drawings of cows and SpongeBobs by bored little kids, but one day we walked in and saw every lyric to Led Zeppelin's "Ramble On" painstakingly transcribed across the entire board.
The genomic location could target de novo methylation to the ICR for example by an RNA that is transcribed across the ICR specifically in male fetal germ cells, similarly to the Gnas DMRs in growing oocytes [69].
The ZmCEL3, ZmCEL11 and ZmCEL14 genes are transcribed across most or all of the internode tissues examined.
We observed that at least 80% of the reference genome assembly was transcribed across all five conditions tested.
Quality information was discarded, and each read transcribed across 6 reading frames into peptide-source elements between STOP codons only (no START codon required).
The 18 genes that were the most differentially transcribed across replicates were analyzed by using RT-PCR to confirm the RNA-seq results.
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DNA microarrays have been extensively used to list differentially transcribed genes across tissues or conditions.
Differentially transcribed genes across samples were identified using the program SAM (Significance Analysis of Microarrays) version 4.0 [ 63].
Since the genes immediately adjacent to the insertion site were not significantly altered, we next identified the most differentially transcribed genes across all replicates.
We have also investigated conservation of potential novel transcribed regions across available nematode species namely: C. elegans, C. briggsae, C. remanei, C. brenneri, Brugia malayi and Pristionchus pacificus.
In conclusion, our experimental approach enabled us to create a comprehensive catalogue of transcribed genes across flower developmental stages and genders.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com