Sentence examples for transcribed according to from inspiring English sources

500 ng of cellular RNA was reverse transcribed according to the manufacturer's directions.

Two mRNA are transcribed according to two different initiation sites (Figure 3a).

For each RNA sample, 2 µg of total RNA was reverse transcribed according to manufacturer's instructions, (Promega).

RNA was prepared from the skin of mouse ears using TRIzol reagent (Invitrogen, Carlsbad, CA) and reverse transcribed according to a published protocol [44].

The cfl1-gfp construct was linearized by NotI, and the capped RNA was transcribed according to the manufacturer's instruction using a mMESSAGE mMACHINE® SP6 Kit (Applied Biosystems, Foster City, CA 94404).

The ptenb construct was linearized by XbaΙ, and the capped RNA was transcribed according to the manufacturer's instruction using a mMESSAGE mMACHINE® T7 Kit (Applied Biosystems, Carlsbad, CA) according to manufacturer's instructions.

cDNA was made by adding 1 μg of the total RNA to the High Capacity RNA-to-cDNA Master Mix (Applied Biosystems, Foster City, CA, USA), and reverse transcribing according to the manufacturer's instructions.

Total RNA was reverse-transcribed according to the manufacturer's instructions (Promega, Catalys AG, Wallisellen, Switzerland).

Using the SuperScript VILO cDNA synthesis kit (Invitrogen) 500 ng of RNA from each sample were reverse-transcribed according to the manufacturer's instructions.

Total RNA, collected as above, was treated with DNase (Applied Biosystems), and reverse-transcribed according to standard protocols.

Next, 200 to 350 ng of RNA was reverse-transcribed according to standard protocols using a Peltier Thermal Cycler PTC-225 (MJ Research).