Sentence examples for trans china from inspiring English sources
The His tag was purified using ProteinIso™ Ni NTA Resin (Trans, China).
The gene (bgl) encoding β-glucosidase was amplified from the template genomic DNA via a polymerase chain reaction (PCR) using DNA polymerase (Trans, China).
The amplified DNA fragment was purified and cloned into a pEASY-E1 6 × His fusion vector using a pEASY-E1 Expression Kit (Trans, China).
The PCR products were purified from the agarose gel using the AxyPrep DNA Gel Extraction Kit (Axygen, China) and cloned into the pEASY-Blunt Zero vector (Trans, China).
The gel blot was probed with anti-Ub (Sigma, Germany) and anti- SUMO1 (Cell Signaling Technology, USA) antibodies and visualized by chemiluminescence using the ECL plus kit (Trans, China) according to the manufacturer's instructions.
To test the differences between the functions of the key genes in L. barbarum and L. ruthenicum, three rate-controlling carotenoid biosynthesis genes from each species (LbPSY1/ LrPSY1, LbCYC-B/ LrCYC-B and LbCRTR-B2/ LrCRTR-B2) were cloned into pEASY-E1 (Trans, China).
Shell is also a partner in the trans-China pipeline, sharing a 15percentt stake with Hong Kong China Gas.
Western analysts have questioned whether that project makes financial sense because of the distances involved, but Beijing has made the trans-China pipeline a strategic priority.
But the trans-China pipeline will not be enough by itself: in announcing today's deal at a press conference here, Wang Fucheng, the executive director of the PetroChina Company, also mentioned that his company had opened talks with Gazprom to import natural gas from Russia as well.
Mark Qiu, the chief financial officer of CNOOC Ltd., a publicly traded unit of CNOOC, said that the Xihu pipeline and the trans-China pipeline could both be completed because there would be enough gas demand in Shanghai to keep both pipelines busy.
Structural analysis along with 40Ar 39Ar and U Pb datings in the Fuping massif provide new insight into the evolution of the eastern part of the Trans-North China Belt (North China Craton), from 2.7 Ga to 1.8 Ga.
