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Carly Jibson ("Hairspray") makes the strongest impression with her powerhouse vocalizing as the camp tramp (2 20).

While TRAMP C1 and C2 cells are tumorigenic when grafted into syngenic C57BL/6J hosts, TRAMP C3 cells are not.

TRAMP C1, C2 and C3 cell lines were originated from a heterogeneous tumor of 32-week TRAMP mouse [30].

Interestingly, the protein level of MBD2 was much higher in TRAMP C1 cells than in TRAMP C3 cells (Figure 4A), suggesting a possible common MBD2-mediated epigenetic suppression of Nrf2 in TRAMP C1 cells.

In contrast, the protein level of AcH3 was apparently lower in TRAMP C1 cells than in TRAMP C3 cells but was increased tremendously by TSA treatment (Figure 4D).

The result reveals that the binding of MBD2 and H3K9m3 to the specific CpGs was substantially higher in TRAMP C1 cells than in TRAMP C3 cells correlating with the fact that the CpGs were minimally methylated in TRAMP C3 cells (Figure 3A).

In current study, we found that the expression of Nrf2 and the induction of NQO1 was compromised in tumorigenic TRAMP C1 cells but not in non-tumorigenic TRAMP C3 cells (Figure 4A).

Here we examined the expression of Nrf2 and one of its downstream target genes NQO1 in TRAMP C1 and C3 cells.

In the current study, ChIP assays were employed to examine the proteins that could be potentially associated with Nrf2 promoter in TRAMP C1 and C3 cells.

Surprisingly, the methylated CpG "hot spots" as found above in the TRAMP prostate tumor were also methylated in tumorigenic C1 cells but not in non-tumorigenic TRAMP C3 cells (Figure 1C).

TRAMP C1 (Tcellsells are derived from TRAMP mice and form tumors when injected orthotopically [ 21].

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