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Furthermore, as the same primary leaf pair was assayed in both treatment groups, a directly comparable developmental trait comparison was made (see Materials and Methods).
The experiments included multiple identical control plots per block for phenotypic trait comparison purposes during data collection (see Table S2 and Table S3).
The experiments included multiple identical control plots per block for disease phenotypic trait comparison purposes during data collection (Table S2 and Table S3).
The experiments included multiple identical control plots per block for phenotypic trait comparison purposes during data collection (i.e. E6203-A and E6203-B) (see Table S2 and Table S3).
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Moreover, because these juveniles died prior to body size re-measurement as well as performance and behavioural testing, the analysis did not include their scores, potentially minimizing the statistical effect of treatments (most trait comparisons yielded non-significant results).
For trait comparisons, we used heterozygotes of the Df 3R ED5579 and DSK001 chromosomes (Df 3R ED5579/DSK001), as well as homozygotes of DSK001 chromosomes (DSK001/DSK001).
The other eight trait comparisons did not show significant correlations.
Of the 72 parent-hybrid trait comparisons among Col, Cvi, and Ws, three had marginally significant HPH.
Genetic differences were detected in three of four trait comparisons for juvenile growth rate and antipredator response.
All previous multi-species studies of trait comparisons between selfers and outcrossers have involved variable mixes of monocarpic and longer-lived polycarpic species.
Again, because we used field-collected seed of DK in this experiment, maternal effects could potentially influence the interpretation of trait comparisons.
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