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This will require greater population sizes and external validation of training profiles and components of profiles in independent populations of subjects with type 1 and type 2 diabetes and should be the focus of a further study.
Thus, a number of training profiles equal to the number of classes was generated.
In addition, the training profiles generated require further validation using independent data sets.
First, using human kidney and liver data from Marioni et al. (2008), we generate expression profiles for each source, both for the (a) training profiles that make up the Source Panel, and (b) for a template healthy patient.
Each query sequence was aligned separately with each of the training profiles using the sequence to profile alignment method in ClustalW 28 and checked for the conservation of cysteines.
47 Although all of the study nurses held bachelor's degrees, we must admit that nursing training profiles in Mexico are heterogeneous, and this could be one of the explanations for our results.
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28 This alignment of representative sequences was used as a training profile for the classification of query sequences.
A training profile is constructed by sampling from the citations selected as relevant for viewing by a user.
Thus, score of a query with the training profile of each class is a measure of its likelihood of belonging to that class.
The number of sequences in the training profile and the number of cysteine positions under consideration vary for the different classes of the protein.
In this study, a cysteine in the query is assumed to be 'strictly conserved' if it aligns perfectly with the cysteine position in the training profile.
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