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This coefficient was 0.72 when just including the eight individually significant trained cells.
Average significant simple spike suppression ranged from 7%to42%2% across 20 trained cells (mean = 20.5%).
Among trained cells, mean percentage simple spike suppression was intimately connected to the prevalence of CS-complex spikes (n = 28, r = 0.804, p < 0.0001; Figure 3A).
In addition, one may also speculate that trained cells present different classes of activation markers than tolerized cells, depending on the subclasses of macrophages induced upon prestimulation.
In line with existing work (e.g., Kotani et al., 2006, Jirenhed et al., 2007), we found a uniquely strong prevalence of simple spike suppression in the trained cells.
In the trained dataset, this criterion adequately separated 28 cells showing this US-complex spike response (henceforth, trained cells; Figures 1C 1E; Table 1) from 12 cells that did not.
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So you need a highly trained cell biologist with a microscope and plenty of time.
Meanwhile, the patient is given a dose of dengue fever, a disease normally carried by mosquitoes, before they are injected with the newly trained dendritic cells.
Immunologically trained T cells, however, have the ability to recognize this infected tissue and kill the virus.
These findings raise an important conceptual question: what is the difference between a stimulated cell, a differentiated macrophage, and a trained immune cell?
The engineered APCs can then be given back to the patient, where they can instruct the patient's T cells as they naturally would, offering a relatively simple way to train T cells to attack cancer cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com