Exact(9)
The length of the trailer region is variable among the members of the subfamily Paramyxovirinae.
The length of the 5'-trailer region of APMV-5 is 552 nt, which is second longest next to APMV-3 (707 nt), whereas the shortest trailer region among the APMV serotypes is 17 nt, for APMV-4.
This suggested that, in the process of divergent evolution by the APMVs the change that was most readily accommodated was in the length of the downstream UTRs and the trailer region.
Each operon has the classical structure of leader region, 16S rRNA coding region, Internal Transcribed Spacer region 1 (ITS1), 23S rRNA coding region, Internal Transcribed Spacer region 2 (ITS2), 5S rRNA coding region and trailer region [2], [3].
The trailer region of APMV-5 is 552 nt in length, which is longer than the typical length (40 60 nt) for most members of the family Paramyxoviridae [44], and is the second longest trailer among APMVs, next to APMV-3 (707 nt) [17].
Instead, the increased genome length of APMV-5 was mostly due to the presence of unusually long 3'(downstream) untranslated regions (UTR) in the P (458 nt), M (531 nt), and HN (378 nt) genes, as well as the presence of a long trailer region (below).
Similar(51)
The 5'UTR region comprises 86 145 nt and contains the trailer regions of size 68 69 nt.
The first 12 nt of the 3' leader sequence of APMV-5 are complementary to the last 12 nt sequence of the 5' trailer regions suggesting the presence of conserved elements in the 3' promoter regions of the genome and antigenome.
We defined 5' leader and 3' trailer regions according to the D. melanogaster release 4.3 annotations.
An analogous transcription termination mechanism was earlier predicted in trailer regions of actively transcribed genes in Actinobacteria [ 31].
We defined the most distal extent of Saccharomyces 5' leader and 3' trailer regions using 5' SAGE data [ 46] as well as expression tiling array data [ 47].
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