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Taken together, the present study demonstrates the antitumour activity of TRAIL in a cytotoxicity (SuperKiller TRAIL) assay and xenograft models (Killer TRAIL) of primary CRC after i.v. administration, which was correlated to DR upregulation and provides estimates of the disposition of TRAIL in humans.
The trail assay was performed as described earlier (Carey et al, 2013).
The "E" trail assay was used mainly to verify that our hexane extracts contained functional aggregation molecules as inputs for GC-MS analyses.
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TRAIL sensitivity assays were conducted using Molecular Probe's Live/Dead Cellular Viability/Cytotoxicity Kit following the infection of breast cancer cells with Ad5hTRAIL.
For example, analysis of breast cancer cell lines by just examining the expression levels of TRAIL death receptors (TRAIL-R1 and TRAIL-R2) and TRAIL-R3 decoy receptor using RNase protection assay did not reveal any connection between the expression pattern of TRAIL receptors and TRAIL resistance [ 28].
The word meant, literally, an "assay", a trail or test or even "experiment".
Motility was assayed by trail deposition visualization, as described [39] with the following modifications.
To determine whether Bid knockdown simply delays cell death or affects long-term survival upon treatment with ABT-737 and TRAIL, we performed colony assays.
In order to investigate the long-term survival effect of FAT1 and caspase-8 depletion, we performed colony forming assays after TRAIL stimulation (Fig 5D).
(He subsequently ascended the best-seller lists with other works in the humorous-travelogue vein, assaying the Appalachian Trail in "A Walk in the Woods" and traversing Australia for "In a Sunburned Country," among others).
Plasma samples were analysed for mapatumumab by an enzyme-linked immunosorbent assay (ELISA) using TRAIL-R1 FLAG TRAIL-R1 FLAGorseradish peroxidase-labelled anti-human lambda antibody detecapture
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