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In those animals receiving only 40 mg/kg D-glucose, no changes in the above-mentioned trafficking parameters were observed (Figures 5A-5D).
In fact, and in contrast with the observations of Booth et al. [44], the mere administration of D-glucose in the absence of the cytokine did not alter leukocyte trafficking parameters.
Actin depolymerization with latrunculin B had no significant effect on trafficking parameters from controls, but it showed significantly greater trafficking parameters, with the exception of directionality index, when compared with astrocytes treated with jasplakinolide to cause actin filament stabilization.
Increasing AR concentration sensitizes to ligand binding and trafficking parameters, as binding and endocytosis become more robust with the larger AR concentration.
Comparing measurements of trafficking dynamics for cells expressing GFP CB1RΔ14aa to the control/full-length GFP CB1R we found that the absence of the C-terminal 14 amino acids resulted in a significant reduction in a subset of trafficking parameters (instantaneous velocity and total track length; Student's t test, P<0.05).
Although it does not appear that long-range movement of CB1R occurs via actin-based mechanisms as observed with D2, since depolymerizing microtubules does not completely abolish active transport and since opposing pharmacological treatments to disrupt or stabilize actin caused significant differences in trafficking parameters, some actin-based trafficking of CB1R may occur.
Similar(53)
Traffic monitoring requires actual traffic parameters.
Triple plot of all traffic parameters.
Different network topologies with different traffic parameters were performed.
Moreover, there are traffic models that perform analysis based on various traffic parameters.
We plotted three observed traffic parameters with their relationships compared with the Greenshields traffic model.
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