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In this respect, the ability of deubiquitinating enzymes (DUBs) to edit the ubiquitination state of protein or to cleave polyubiquitin chains from substrates is a key step in the correct definition of tags for subcellular localization and intracellular trafficking of target protein [ 21].
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Time-lapse imaging has emerged as a powerful tool for investigating dynamic cellular processes such as cell division or intracellular trafficking of labeled targets of interest.
The NTT2-mCherry marker consistently labelled the perimeter of individual chloroplasts, indicating that the trafficking of proteins targeted to the chloroplast envelope was not impaired in the leaf protoplast transient expression system.
Our findings signify the importance of the cellular trafficking properties of targeting ligands, as they may significantly influence therapeutic potency when such ligands are conjugated to NPs.
Communication traffic of the target SoC is analyzed (by means of analytic calculations and simulations), and QoS requirements (delay and throughput) for each service class are derived.
Finally, we note non-affinity aspects to uptake and intracellular trafficking of carriers in target cells.
In hepatocytes, FA are taken up by transporters [ 45], followed by poorly defined trafficking of FA to target organelles such as mitochondria and peroxisomes to undergo oxidation.
In their study, Estes et al. demonstrated that dFMRP shuttling promotes trafficking of its mRNA targets between RNA neuronal granules and the cytosolic fraction (Estes et al., 2008).
Our data do not exclude the possibility that Rab21 is required for the delivery or trafficking of other critical targets, in addition to integrin α5.
There are no effective drugs in use that target trafficking of VGSC.
Glucocorticoids suppress DC, monocytes and lymphocytes, with reduction of trafficking of proinflammatory cells to target tissues, but they are quite toxic.
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