In this situation, trading specificity for high sensitivity is desirable because missing events of international public health concern would have serious consequences and would outweigh benefits of a lower volume of false-positive results.
Also, we extend its functionality to allow users to trade off specificity and sensitivity by setting different decision thresholds.
Trading off specificity for sensitivity, i.e., requiring a single read on one strand and two on the other, detects three additional true variants, but also 11 suspected false positives not reported by GA.
Besides the obvious improvement, we can also observe the fluctuation of the curves: theoretically sensitivity can be traded for specificity and vice versa, so that the ROC curves should be in "smooth" shapes, which is not the case for Fig. 1.
Of course, judiciously trading off specificity for sensitivity is a standard and sound practice in statistical data analysis, however, by increasing the number of false positives, ENCODE achieves an increase in the total number of test positives, thereby, exaggerating the fraction of "functional" elements within the genome.
One must be wary of trading potency for specificity, as targeting very specific amino acid sequences in highly mutated tumors may result in killing only a subgroup of cells.
However, the sensitivity and specificity trade off with each other.
UCHIME reports a score for each sequence, allowing the user to trade sensitivity for specificity by adjusting the minimum score threshold used to discriminate chimeras from biological sequences.
By tuning alignment indexing and min-identity parameters, READSCAN's high-sensitive mode achieved higher sensitivity with a trade off in specificity and time (Fig. 2).
We improve DISpro to allow users to set different thresholds to trade off the specificity and sensitivity of disorder predictions and to add a function for the visualization of protein disorder prediction.
