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Most intriguingly, distinct tumor margins, even the tracks of migration of small number of tumor cells, were identified based on the fluorescence images, which correlated well with histological staining of the tumor (Fig. 5).
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Fig. 1 Schematic representation of the in vivo tracking of DC migration by performing multimodal reporter gene imaging.
For example, long term tracking of animal migration over large scales can be accomplished with the lower accuracy geolocation method [29], [30].
The phospho-glycerate kinase (PGK) promoter was used to ubiquitously direct expression of histone-2B fused eGFP and mCherry proteins to the nucleus to monitor DNA content and enable tracking of cell migration and lineage.
A major advantage of this system is the tagging of a fluorescent marker to the gene of interest, enabling powerful real-time tracking of lymphocyte migration and proliferation.
A 100 bp DNA ladder (Promega Cooperation, USA) and Orange 6× loading dye were also used for visual tracking of DNA migration during electrophoresis.
The vertical cleavage plane may derail them from the outward-bound track of radial migration, confine them to the malleable ventricular zone and benefit the laminar assembly of ablated neocortex by sparing the marginal and mantle zones from the clearance of excessive killed cells.
The gel was subsequently stained and photographed (fluorescent ruler of gel-casting tray was used to track the distance of migration of DNA bands).
'n' indicates the number of migration tracks analysed.
Ornithologists rely on bird-watchers to keep track of changing patterns of migration, astronomers have long profited from enthusiasts scanning the skies to spot new comets, and archaeologists benefit from amateurs' finds.
Edn3 mRNA appears to be expressed in a spatially and temporally regulated manner that tracks the migration of ENCDCs, and EDNRB signaling is required during a very narrow temporal window roughly corresponding to colonic migration.
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