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We used a FACS-based method tracking expression of the erythroid-specific antigen TER-119 and CD71 (transferrin receptor) [29], [30] to compare normal and Sod2-/ erythroid differentiation (figure 1A) in murine bone marrow.
By tracking expression of lineage determining transcription factors during positive selection, we now report that the Cd8 coreceptor gene locus co-opts any coreceptor protein encoded within it to induce thymocytes to express the cytotoxic-lineage factor Runx3 and to adopt the cytotoxic-lineage fate, findings we refer to as 'coreceptor gene imprinting'.
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In parallel, media samples were taken to track expression of construct 4 by PAGE and western blot (Fig. 5A, B).
Some spontaneous fluorescence proteins have been used as a reporter gene to track expression of certain genes.
The gene-transfer plasmid (pHIV-ZsGreen, Addgene plasmid 18121) encodes a GFP from the reef coral Zoanthus to track expression of the transgene in infected cells.
To evaluate bladder regeneration, the dogs were sacrificed and their bladders were examined macroscopically and microscopically for presence of tracking proteins, expression of cell-specific markers and histological attributes of regenerated tissues.
Applicability of the assay was evaluated by tracking protein expression of two GST-fused allergens of biopharmaceutical value in E. coli, GST-Der p 2 and GST-Mus a 5, both targeted to inclusion bodies.
To fit the puzzle together, a team led by Catherine Thompson, a molecular biologist at the Kennedy Krieger Institute and Johns Hopkins University School of Medicine in Baltimore, Maryland, started by tracking the expression of Hairless in healthy follicles.
Tracking the expression of the transferred TCR is a critical issue to determine the frequency of transduced T cells and the degree of mispairing between the transferred and the endogenous TCR α and β chains.
We examined HDAC2 activity in THI-treated mdx muscles by tracking the expression of its target genes, especially miR-1 and miR-29.
We capture spikes in fear in different cities by using sentiment and time-series analyses, and track expressions of sympathy and solidarity based on the emergent use of two hashtags, #prayforboston and #bostonstrong, widely adopted after the bombings.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com