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This new paradigm requires different lead optimization, different types of analysis, and quantitation.
The single-molecule, 2D diffusion rates of both constructs on PC/PS supported bilayers were measured by TIRF imaging and quantitation of hundreds of single-particle diffusion tracks as previously described.
Routine DNA extraction and quantitation is readily available.
The limits of detection and quantitation were 0.8 and 2.30 ng/mL, respectively.
Image degradation may include poorer lesion detectability and inaccuracy in location, volume definition, and quantitation.
Fig. 2 HPLC analysis and quantitation of DML, MJ, and lovastatin titers of the engineered strains.
The limits of detection and quantitation for the method were 2.9 and 8.7 ng/mL, respectively.
The limits of detection and quantitation were 15.5 and 51.2 μg mL−1, respectively.
The limits of detection and quantitation were 5.3 and 15.8 μg/well, respectively.
The limit of detection (LOD) and quantitation (LOQ) were determined by making serials of dilutions.
Method validation parameters included linearity, recovery, repeatability, precision, stability and quantitation limit.
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